Cht ceramic hydroxyapatite manual

Section 1. Hydroxyapatite, a crystalline form of calcium phosphate, is widely used in preparative biochemistry, having proven itself a unique tool for the fractionation and purification of monoclonal antibodies and other proteins, 8,9 enzymes, and nucleic acids.

Hydroxyapatite is useful for preparative work in. If you have any questions or comments regarding these CHT ceramic hydroxyapatite is a rigid support and can operate under high flow rates and pressures. This mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT media for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.

The robust properties of CHT Ceramic Hydroxyapatite improve efficiency, yield, and financial value through:. Both types have elution characteristics similar to crystalline hydroxyapatite but also have some important differences:. These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results.

Please download the CHT Application Guide for specific details on recommended buffers and slurry calculations. Watch Videos Download Application Guide. The unique separation properties and selectivity of CHT Media enable you to maximize purity and recovery in a single step of downstream purification for the development of biotherapeutics. Watch Video. Regulatory support files are available upon request. You can create and edit multiple shopping carts Edit mode — allows you to edit or modify an existing requisition prior to submitting.

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Bio-Rad Products Explore all. Variance in specifications and construction of the columns should be taken into consideration when packing your own column. Agitate the buffer while de-aerating with nitrogen for 10 min. Prime the line to the column with 10 L of buffer. Mix for an additional 10 min following the addition of the 29th CHT container while closing and sealing the column hatch.

Pressurize the tank to 45 psi with nitrogen gas and hold for 1 min. Open the tank valve to fill the column while maintaining 45 psi tank pressure until the packing nozzle on the column stalls.

Immediately close the packing nozzle. Close the tank valve and release the pressure on the tank. Disconnect the line from the slurry tank at the column filling connection and direct it to a collection container.

Open the tank valve, then pressurize the tank if necessary to empty it of excess slurry. Turn off the nitrogen line if used in the prior step. Rinse the interior with WFI with the tank spraying system, collecting the contents in the collection container.

Flush and clean the filling line according to the instructions in the Chromaflow column and packing station instruction manuals. Connect the column to the process chromatography workstation.